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Cryptococcus gattii in urban trees from cities in North‐eastern Argentina
Author(s) -
Mazza Mariana,
Refojo Nicolás,
BoscoBorgeat María Eugenia,
Taverna Constanza Giselle,
Trovero Alicia Cristina,
Rogé Ariel,
Davel Graciela
Publication year - 2013
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12084
Subject(s) - cryptococcus gattii , amplified fragment length polymorphism , biology , serotype , genotype , veterinary medicine , cryptococcus , microbiology and biotechnology , genetic diversity , medicine , population , genetics , gene , environmental health
Summary In the city of Buenos Aires, Argentina, Cryptococcus gattii genotype AFLP 4/ VGI was found to be associated with decaying wood in hollows of different tree species. The aim of this study was to investigate the presence of C. gattii in the environment of riverside cities of the river Paraná, and to describe its serotypes and molecular types. Five hundred samples were collected in 50 parks by swabbing tree hollows. The samples were inoculated on caffeic acid agar supplemented with chloramphenicol, and incubated at 28 °C for 1 week with a daily observation. The isolates were identified by conventional methods. The serotype was determined by slide agglutination with specific antisera. Molecular typing was carried out by PCR ‐ RFLP of the URA 5 gene. Four isolates of C. gattii were recovered: Cryptococcus gattii serotype B, genotype AFLP 4/ VGI , isolated from Eucalyptus sp. in the city of Rosario and from Grevillea robusta in the city of La Paz; and C. gattii serotype C, genotype AFLP 5/ VGIII , isolated from two different Tipuana tipu trees in the city of Resistencia. Here, we report for the first time the isolation of C. gattii serotype C, genotype AFLP 5/ VGIII , from environmental samples in Argentina.