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Sequence and transcript expression of the super ‐kdr locus of the horn fly, Haematobia irritans
Author(s) -
Domingues L. N.,
Solis G. D.,
Bendele K. G.,
Foil L. D.,
Perez de Leon A. A.,
Guerrero F. D.
Publication year - 2020
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12442
Subject(s) - haematobia irritans , biology , muscidae , locus (genetics) , genetics , genomic dna , allele , rna splicing , pyrethroid , gene , genotype , rna , zoology , ecology , stomoxys , pesticide
In horn flies, Haematobia irritans irritans (Diptera: Muscidae) (Linnaeus, 1758), target site resistance to pyrethroids can be diagnosed by an allele‐specific PCR that genotypes individual flies at both the super‐kdr (skdr) and the knock down resistance (kdr) associated loci. When this technique uses genomic DNA as template, modifications, such as alternative RNA splicing and RNA editing are not specifically detected. Alternative splicing at the skdr locus has been reported in Dipterans; thus, the genomic DNA‐based allele‐specific PCR may not accurately reflect the frequency of the skdr mutation in horn fly field populations. To investigate if alternative splicing occurs at the skdr locus of horn flies, genomic DNA and cDNA sequences isolated from two wild populations and two laboratory‐reared colonies with varying degrees of pyrethroid resistance were compared. There was no indication of alternative splicing at the super‐kdr locus neither in the wild populations nor in the laboratory‐reared colonies.