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Application of hydrolysis probe analysis to identify clade types of the malaria vector mosquito Anopheles funestus sensu stricto from M uheza, northeastern T anzania
Author(s) -
KWEKA E. J.,
MAUSA E. A.,
VENTER N.,
DERUA Y. A.,
KIMARO E. E.,
COETZEE M.
Publication year - 2018
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12274
Subject(s) - clade , biology , sensu stricto , sensu , anopheles , vector (molecular biology) , tanzania , malaria , zoology , species complex , veterinary medicine , genus , phylogenetics , genetics , gene , immunology , phylogenetic tree , environmental planning , recombinant dna , medicine , environmental science
A hydrolysis probe analysis (TaqMan assay) was used to study clade types in Anopheles funestus sensu stricto Giles, a major malaria vector in sub‐Saharan Africa, with specimens collected from Muheza in Tanga, northeastern Tanzania. A total of 186 An. funestus specimens were analysed, revealing that 176 (94.6%) were of clade I and 10 (5.4%) of clade II. These findings extend the distribution of clade type II from southern Mozambique and northern Zambia to northeastern Tanzania. The technique used can also be of great value in assessing the role and contribution of these clade types in malaria transmission and insecticide resistance frequencies for An. funestus s.s .