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Molecular evidence for trans‐stadial transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato under field conditions
Author(s) -
AKTAS M.,
OZUBEK S.
Publication year - 2018
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12261
Subject(s) - rhipicephalus sanguineus , biology , nymph , ixodidae , sensu , veterinary medicine , tick , zoology , virology , genus , medicine
This study investigated the transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato ( Ixodida: Ixodidae ) . Engorged nymphs ( n = 404) removed from A. platys ‐infected dogs were incubated at 28 °C until moulting. Unfed adults were obtained and divided into 48 pools. Unfed ticks collected from the grounds of the dog shelter, comprising 1800 larvae ( n = 18 pools), 3100 nymphs ( n = 62 pools) and 85 adults ( n = 10 pools, including three male and seven female pools) were sorted into 90 pools. All pools were screened by polymerase chain reaction for the 16S r RNA gene of A. platys . Of 48 pools of unfed adults obtained from engorged nymphs, 12 were positive for A. platys ; the infection rate maximum likelihood estimate ( MLE ) was 3.36 [95% confidence interval ( CI) 1.84–5.68]. Anaplasma platys was detected in five of 23 male pools ( MLE 2.82, 95% CI 1.06–6.20) and seven of 25 female pools ( MLE 3.83, 95% CI 1.72–7.57). Of seven pools of unfed adult females collected from the shelter grounds, one was positive for A. platys ( MLE 1.74, 95% CI 0.11–8.22). Among 62 unfed nymph pools, eight were infected with A. platys ( MLE 0.27, 95% CI 0.13–0.52). No A. platys DNA was detected in the larva pools. The present results reveal molecular evidence for the trans‐stadial transmission of A. platys by R. sanguineus s.l.