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Sperm dimorphism in the T riatoma brasiliensis species complex and its applications
Author(s) -
BAFFA A. F.,
CAMARA D. C. P.,
SANTOSMALLET J. R.,
DA SILVA E. R.,
COSTA J.,
FREITAS S. P. C.
Publication year - 2017
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12226
Subject(s) - biology , sperm , ultrastructure , zoology , synapomorphy , phylogenetics , botany , genetics , gene , clade
Morphological and structural features of the sperm of the T riatoma brasiliensis N eiva, 1911 ( H emiptera: R eduviidae) species complex were examined in this first study investigating the sperm of H eteroptera and the genus T riatoma . Males were dissected and their seminal vesicles removed. For measurement, seminal vesicles were squashed on glass slides, spread, fixed and observed under a photomicroscope. The images were analysed and measures of sperm were made. Data were analysed using one‐way analysis of variance and T ukey's test to detect differentiation among taxa. Furthermore, seminal vesicles were prepared for studies of transmission electron microscopy. All taxa studied showed polymorphic (short and long) sperm. The sperm of T riatoma brasiliensis macromelasoma was significantly longer (in total length) than that of the other four members of the complex, which supports the hypothesis of hybrid speciation of this member of the complex as an increase in the size of typical hybrids under heterosis was previously shown. The sperm cells of the five taxa have similar ultrastructural morphology. The ultrastructural features observed confirm the hypothesis, raised by previous studies, that they are synapomorphic to the suborder H eteroptera.