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The recombinant protein r SP03B is a valid antigen for screening dog exposure to P hlebotomus perniciosus across foci of canine leishmaniasis
Author(s) -
KOSTALOVA T.,
LESTINOVA T.,
MAIA C.,
SUMOVA P.,
VLKOVA M.,
WILLEN L.,
POLANSKA N.,
FIORENTINO E.,
SCALONE A.,
OLIVA G.,
VERONESI F.,
CRISTÓVÃO J. M.,
COURTENAY O.,
CAMPINO L.,
GRADONI L.,
GRAMICCIA M.,
VOLF P.
Publication year - 2017
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12192
Subject(s) - biology , sandfly , virology , vector (molecular biology) , antigen , antibody , leishmania infantum , psychodidae , xenodiagnosis , canine leishmaniasis , recombinant dna , leishmaniasis , leishmania , immunology , parasite hosting , visceral leishmaniasis , trypanosoma cruzi , genetics , world wide web , computer science , gene
Abstract The frequency of sandfly–host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate ( SGH ); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. P hlebotomus perniciosus ( D iptera: P sychodidae) is the main vector of L eishmania infantum ( T rypanosomatida: T rypanosomatidae) in southwest E urope and is widespread from P ortugal to I taly. In this study, sera were sampled from naturally exposed dogs from distant regions, including C ampania (southern I taly), U mbria (central I taly) and the metropolitan L isbon region ( P ortugal), where P . perniciosus is the unique or principal vector species. Sera were screened for anti‐ P . perniciosus antibodies using SGH and 43‐ kDa yellow‐related recombinant protein ( rSP03B ). A robust correlation between antibodies recognizing SGH and rSP03B was detected in all regions, suggesting substantial antigenic cross‐reactivity among different P . perniciosus populations. No significant differences in this relationship were detected between regions. Moreover, rSP03B and the native yellow‐related protein were shown to share similar antigenic epitopes, as canine immunoglobulin G ( IgG ) binding to the native protein was inhibited by pre‐incubation with the recombinant form. These findings suggest that rSP03B should be regarded as a universal marker of sandfly exposure throughout the geographical distribution of P . perniciosus .