Premium
Comparative analysis of midgut bacterial communities in three aedine mosquito species from dengue‐endemic and non‐endemic areas of R ajasthan, I ndia
Author(s) -
CHARAN S. S.,
PAWAR K. D.,
GAVHALE S. D.,
TIKHE C. V.,
CHARAN N. S.,
ANGEL B.,
JOSHI V.,
PATOLE M. S.,
SHOUCHE Y. S.
Publication year - 2016
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12173
Subject(s) - biology , dengue fever , midgut , aedes aegypti , aedes albopictus , vector (molecular biology) , phylotype , polymerase chain reaction , 16s ribosomal rna , zoology , bacteria , virology , ecology , genetics , gene , larva , recombinant dna
Dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. Differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. To investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely S tegomyia aegypti (= A edes aegypti ), F redwardsius vittatus (= A edes vittatus ) and S tegomyia albopicta (= A edes albopictus ) (all: D iptera: C ulicidae), from dengue‐endemic and non‐endemic areas of R ajasthan, I ndia were compared. Construction and analyses of six 16S rRNA gene libraries indicated that S erratia spp.‐related phylotypes dominated all clone libraries of the three mosquito species from areas in which dengue is not endemic. In dengue‐endemic areas, phylotypes related to A eromonas , E nhydrobacter spp. and uncultivated bacterium dominated the clone libraries of S. aegypti , F . vittatus and S . albopicta , respectively. Diversity indices analysis and real‐time TaqMan polymerase chain reaction assays showed bacterial diversity and abundance in the midguts of S. aegypti to be higher than in the other two species. Significant differences observed among midgut bacterial communities of the three mosquito species from areas in which dengue is and is not endemic, respectively, may be related to the vectorial capacity of mosquitoes to carry dengue viruses and, hence, to the prevalence of disease in some areas.