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Differential protein expression in the midgut of C ulex quinquefasciatus mosquitoes induced by the insecticide temephos
Author(s) -
GAMES P. D.,
ALVES S. N.,
KATZ B. B.,
TOMICH J. M.,
SERRÃO J. E.
Publication year - 2016
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12172
Subject(s) - midgut , biology , culex quinquefasciatus , proteome , chikungunya , filariasis , aedes aegypti , anopheles gambiae , microbiology and biotechnology , virology , dengue fever , immunology , larva , botany , bioinformatics , malaria , helminths
Mosquitoes are vectors for pathogens of malaria, lymphatic filariasis, dengue, chikungunya, yellow fever and J apanese encephalitis. C ulex quinquefasciatus S ay, 1823 ( D iptera: C ulicidae) is a known vector of lymphatic filariasis. Its control in Brazil has been managed using the organophosphate temephos. Studies examining the proteins of C x. quinquefasciatus that are differentially expressed in response to temephos further understanding of the modes of action of the insecticide and may potentially identify resistance factors in the mosquito. In the present study, a comparative proteomic analysis, using 2‐dimensional electrophoresis coupled with matrix‐assisted laser desorption/ionization ( MALDI ) time of flight ( TOF )/ TOF mass spectrometry, and bioinformatics analyses were performed to identify midgut proteins in C x. quinquefasciatus larvae that were differentially expressed in response to exposure to temephos relative to those in untreated controls. A total of 91 protein spots were differentially expressed; 40 were upregulated and 51 were downregulated by temephos. A total of 22 proteins, predominantly upregulated, were identified as known to play a role in the immune response, whereas the downregulated proteins were involved in energy and protein catabolism. This is the first proteome study of the midgut of C x. quinquefasciatus and it provides insights into the molecular mechanisms of insecticide‐induced responses in the mosquito.