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Development and evaluation of real‐time PCR assays for bloodmeal identification in Culicoides midges
Author(s) -
VAN DER SAAG M. R.,
GU X.,
WARD M. P.,
KIRKLAND P. D.
Publication year - 2016
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12163
Subject(s) - ceratopogonidae , biology , culicoides , host (biology) , vector (molecular biology) , identification (biology) , transmission (telecommunications) , zoology , polymerase chain reaction , ecology , virology , genetics , gene , engineering , electrical engineering , recombinant dna
Culicoides (Diptera: Ceratopogonidae) midges are the biological vectors of a number of arboviruses of veterinary importance. However, knowledge relating to the basic biology of some species, including their host‐feeding preferences, is limited. Identification of host‐feeding preferences in haematophagous insects can help to elucidate the transmission dynamics of the arboviruses they may transmit. In this study, a series of semi‐quantitative real‐time polymerase chain reaction (qPCR) assays to identify the vertebrate host sources of bloodmeals of Culicoides midges was developed. Two pan‐reactive species group and seven species‐specific qPCR assays were developed and evaluated. The assays are quick to perform and less expensive than nucleic acid sequencing of bloodmeals. Using these assays, it was possible to rapidly test nearly 700 blood‐fed midges of various species from several geographic locations in Australia.