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A multi‐locus approach resolves the phylogenetic relationships of the Simulium asakoae and Simulium ceylonicum species groups in Malaysia: evidence for distinct evolutionary lineages
Author(s) -
LOW V. L.,
TAKAOKA H.,
ADLER P. H.,
YA'COB Z.,
NORMARASHID Y.,
CHEN C. D.,
SOFIANAZIRUN M.
Publication year - 2015
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12120
Subject(s) - biology , simulium , phylogenetic tree , locus (genetics) , subgenus , ribosomal rna , phylogenetics , gene , genetics , mitochondrial dna , cytochrome c oxidase subunit i , black fly , evolutionary biology , zoology , taxonomy (biology) , ecology , larva
A multi‐locus approach was used to examine the DNA sequences of 10 nominal species of blackfly in the S imulium subgenus Gomphostilbia ( D iptera: S imuliidae) in M alaysia. Molecular data were acquired from partial DNA sequences of the mitochondria‐encoded cytochrome c oxidase subunit I ( COI ), 12S rRNA and 16S rRNA genes, and the nuclear‐encoded 18S rRNA and 28S rRNA genes. No single gene, nor the concatenated gene set, resolved all species or all relationships. However, all morphologically established species were supported by at least one gene. The multi‐locus sequence analysis revealed two distinct evolutionary lineages, conforming to the morphotaxonomically recognized Simulium asakoae and Simulium ceylonicum species groups.