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Detection and isolation of the α‐proteobacterium Asaia in Culex mosquitoes
Author(s) -
DE FREECE C.,
DAMIANI C.,
VALZANO M.,
D'AMELIO S.,
CAPPELLI A.,
RICCI I.,
FAVIA G.
Publication year - 2014
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/mve.12045
Subject(s) - biology , anopheles stephensi , aedes aegypti , culex quinquefasciatus , aedes , anopheles gambiae , culex pipiens , anopheles , culex , aedes albopictus , context (archaeology) , zoology , vector (molecular biology) , ecology , malaria , larva , genetics , immunology , gene , recombinant dna , paleontology
Investigations of microbiota within mosquitoes continue to widen the spectrum of possible symbiont‐based applications against vector‐borne diseases. In this context, α‐proteobacteria of the genus Asaia (Rhodospirillales: Acetobacteraceae) are emerging as possible endosymbiotic candidates, particularly in paratransgenic approaches aimed at interrupting pathogen transmission. Previous studies have shown that Asaia spp. distribution among Anopheles gambiae and Anopheles stephensi (Diptera: Culicidae) mosquitoes displayed positive rates of infection in isolated midguts, salivary glands and reproductive tissues. Similarly, Asaia has been detected in Aedes albopictus ( Stegomyia albopicta ) and Aedes aegypti ( Stegomyia aegypti ) (Diptera: Culicidae) populations. Within the Culex pipiens complex (Diptera: Culicidae), Asaia infection is still largely unexplored. Here, we summarize a preliminary survey of laboratory‐reared Cx. pipiens complex and field‐collected Culex quinquefasciatus for the presence of Asaia spp., and present the first identification of Asaia in some of the members of the Cx. pipiens complex and the first description in W est A frican populations of Cx. quinquefasciatus .