Open AccessAn ERF121 transcription factor from Brassica oleracea is a target for the conserved TAL‐effectors from different Xanthomonas campestris pv. campestris strains
Author(s) -
Zlobin Nikolay,
Lebedeva Marina,
Monakhova Yuliya,
Ustinova Vera,
Taranov Vasiliy
Publication year - 2021
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.13048
Subject(s) - xanthomonas campestris , xanthomonas campestris pv. campestris , biology , xanthomonas , effector , brassica oleracea , gene , genetics , brassica , transcription factor , genome , arabidopsis , microbiology and biotechnology , botany , mutant
Transcription activator‐like effectors (TALEs), which induce the expression of specific plant genes to promote infection, are the main pathogenic determinants of various Xanthomonas bacteria. However, investigation of TALEs from Xanthomonas campestris pv. campestris , which causes black rot disease of crucifers, received little attention. In this study, we used PCR‐based amplification followed by SMRT amplicon sequencing to identify TALE genes in several X . campestris pv. campestris strains. Computational prediction in conjunction with quantitative reverse transcription PCR analysis was used to find their targets in the Brassica oleracea genome. Transcription factor ERF121, from the AP2/ERF family, was identified as target gene for the conserved TALEs from multiple X . campestris pv. campestris strains. Several members of this family from diverse plants were previously identified as targets of TALEs from different Xanthomonas species. We propose that TALE‐dependent activation of AP2/ERF transcription factors promotes susceptibility to Xanthomonas through the misregulation of plant defence pathways.