Open AccessIdentification of viral particles in the apoplast of Nicotiana b enthamiana leaves infected by potato virus X
Author(s) -
Hu Shuzhen,
Yin Yueyan,
Chen Buyang,
Lin Qi,
Tian Yanzhen,
Song Xijiao,
Peng Jiejun,
Zheng Hongying,
Rao Shaofei,
Wu Guanwei,
Mo Xiaohan,
Yan Fei,
Chen Jianping,
Lu Yuwen
Publication year - 2021
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.13039
Subject(s) - apoplast , nicotiana benthamiana , potato virus x , biology , plant virus , western blot , virus , potexvirus , extracellular , virology , botany , biochemistry , cell wall , rna , coat protein , gene
The apoplast is the extracellular space for signalling, nutrient transport, and plant–microbe interactions, but little is known about how plant viruses use the foliar apoplast. Proteomic analysis of the apoplasts isolated from potato virus X (PVX)‐infected Nicotiana benthamiana plants showed that the coat protein (CP) is the dominant viral component. The presence of the CP in the apoplast was confirmed by western blot, viral nucleic acid was detected by reverse transcription‐PCR and northern blot, and viral particles were observed by transmission electron microscopy (TEM). The apoplast from infected leaves was infectious if rubbed onto healthy leaves but not when infiltrated into them. The exosomes were separated from the apoplast fluid by high‐speed centrifugation and TEM showed that PVX particles were not associated with the exosomes. These results suggest that PVX virions are released to the N. benthamiana apoplast in a one‐way manner and do not share the bidirectional transport of exosomes.