Open AccessSoybean RNA interference lines silenced for eIF4E show broad potyvirus resistance
Author(s) -
Gao Le,
Luo Jinyan,
Ding Xueni,
Wang Tao,
Hu Ting,
Song Puwen,
Zhai Rui,
Zhang Hongyun,
Zhang Kai,
Li Kai,
Zhi Haijian
Publication year - 2020
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12897
Subject(s) - potyvirus , soybean mosaic virus , biology , potyviridae , tobacco etch virus , rna interference , virology , potato virus y , bimolecular fluorescence complementation , genetically modified crops , cultivar , virus , plant virus , untranslated region , mosaic virus , genetics , transgene , rna , botany , gene
Soybean mosaic virus (SMV), a potyvirus, is the most prevalent and destructive viral pathogen in soybean‐planting regions of China. Moreover, other potyviruses, including bean common mosaic virus (BCMV) and watermelon mosaic virus (WMV), also threaten soybean farming. The eukaryotic translation initiation factor 4E (eIF4E) plays a critical role in controlling resistance/susceptibility to potyviruses in plants. In the present study, much higher SMV‐induced eIF4E1 expression levels were detected in a susceptible soybean cultivar when compared with a resistant cultivar, suggesting the involvement of eIF4E1 in the response to SMV by the susceptible cultivar. Yeast two‐hybrid and bimolecular fluorescence complementation assays showed that soybean eIF4E1 interacted with SMV VPg in the nucleus and with SMV NIa‐Pro/NIb in the cytoplasm, revealing the involvement of VPg, NIa‐Pro, and NIb in SMV infection and multiplication. Furthermore, transgenic soybeans silenced for eIF4E were produced using an RNA interference approach. Through monitoring for viral symptoms and viral titers, robust and broad‐spectrum resistance was confirmed against five SMV strains (SC3/7/15/18 and SMV‐R), BCMV, and WMV in the transgenic plants. Our findings represent fresh insights for investigating the mechanism underlying eIF4E‐mediated resistance in soybean and also suggest an effective alternative for breeding soybean with broad‐spectrum viral resistance.