Open AccessThe DnaJ protein OsDjA6 negatively regulates rice innate immunity to the blast fungus Magnaporthe oryzae
Author(s) -
Zhong Xionghui,
Yang Jiuxia,
Shi Yanlong,
Wang Xuli,
Wang GuoLiang
Publication year - 2018
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12546
Subject(s) - biology , magnaporthe , genetically modified rice , innate immune system , oryza sativa , plant disease resistance , ubiquitin ligase , pyricularia , microbiology and biotechnology , rna interference , xanthomonas oryzae , transgene , genetically modified crops , botany , gene , pathogen , magnaporthe grisea , genetics , ubiquitin , immune system , rna
Summary Rice blast, caused by Magnaporthe oryzae (synonym: Pyricularia oryzae ), severely reduces rice production and grain quality. The molecular mechanism of rice resistance to M. oryzae is not fully understood. In this study, we identified a chaperone DnaJ protein, OsDjA6, which is involved in basal resistance to M. oryzae in rice. The OsDjA6 protein is distributed in the entire rice cell. The expression of OsDjA6 is significantly induced in rice after infection with a compatible isolate. Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments. In addition, the expression levels of WRKY45 , NPR1 and PR5 are increased in OsDjA6 RNAi plants, indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway. Finally, we found that OsDjA6 interacts directly with the E3 ligase OsZFP1 in vitro and in vivo . These results suggest that the DnaJ protein OsDjA6 negatively regulates rice innate immunity, probably via the ubiquitination proteasome degradation pathway.