Open AccessComparative proteomic analysis of Xanthomonas citri ssp. citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction
Author(s) -
Artier Juliana,
da Silva Zandonadi Flávia,
de Souza Carvalho Flávia Maria,
Pauletti Bianca Alves,
Leme Adriana Franco Paes,
Carnielli Carolina Moretto,
SelistredeAraujo Heloisa Sobreiro,
Bertolini Maria Célia,
Ferro Jesus Aparecido,
Belasque Júnior José,
de Oliveira Julio Cezar Franco,
NovoMansur Maria Teresa Marques
Publication year - 2018
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12507
Subject(s) - biology , xanthomonas , citrus canker , xanthomonas citri , virulence , microbiology and biotechnology , periplasmic space , biochemistry , phosphoglucomutase , ftsz , hypersensitive response , pathogen , bacteria , escherichia coli , enzyme , genetics , gene , plant disease resistance
Summary Citrus canker is a plant disease caused by Gram‐negative bacteria from the genus Xanthomonas . The most virulent species is Xanthomonas citri ssp. citri (XAC), which attacks a wide range of citrus hosts. Differential proteomic analysis of the periplasm‐enriched fraction was performed for XAC cells grown in pathogenicity‐inducing (XAM‐M) and pathogenicity‐non‐inducing (nutrient broth) media using two‐dimensional electrophoresis combined with liquid chromatography‐tandem mass spectrometry. Amongst the 40 proteins identified, transglycosylase was detected in a highly abundant spot in XAC cells grown under inducing condition. Additional up‐regulated proteins related to cellular envelope metabolism included glucose‐1‐phosphate thymidylyltransferase, dTDP‐4‐dehydrorhamnose‐3,5‐epimerase and peptidyl‐prolyl cis – trans ‐isomerase. Phosphoglucomutase and superoxide dismutase proteins, known to be involved in pathogenicity in other Xanthomonas species or organisms, were also detected. Western blot and quantitative real‐time polymerase chain reaction analyses for transglycosylase and superoxide dismutase confirmed that these proteins were up‐regulated under inducing condition, consistent with the proteomic results. Multiple spots for the 60‐kDa chaperonin and glyceraldehyde‐3‐phosphate dehydrogenase were identified, suggesting the presence of post‐translational modifications. We propose that substantial alterations in cellular envelope metabolism occur during the XAC infectious process, which are related to several aspects, from defence against reactive oxygen species to exopolysaccharide synthesis. Our results provide new candidates for virulence‐related proteins, whose abundance correlates with the induction of pathogenicity and virulence genes, such as hrpD6 , hrpG , hrpB7 , hpa1 and hrpX . The results present new potential targets against XAC to be investigated in further functional studies.