Open AccessA novel leucine‐rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection
Author(s) -
Cheng Wei,
Xiao Zhuoli,
Cai Hanyang,
Wang Chuanqing,
Hu Yang,
Xiao Yueping,
Zheng Yuxing,
Shen Lei,
Yang Sheng,
Liu Zhiqin,
Mou Shaoliang,
Qiu Ailian,
Guan Deyi,
He Shuilin
Publication year - 2017
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12462
Subject(s) - ralstonia solanacearum , pepper , jasmonic acid , biology , systemic acquired resistance , gene silencing , transgene , gene , microbiology and biotechnology , ectopic expression , genetically modified crops , regulator , salicylic acid , hypersensitive response , plant defense against herbivory , plant disease resistance , genetics , pathogen , arabidopsis , horticulture , mutant
Summary The leucine‐rich repeat (LRR) proteins play important roles in the recognition of corresponding ligands and signal transduction networks in plant defence responses. Herein, a novel LRR protein from Capsicum annuum , CaLRR51, was identified and characterized. It was localized to the plasma membrane and transcriptionally up‐regulated by Ralstonia solanacearum infection (RSI), as well as the exogenous application of salicylic acid (SA), jasmonic acid (JA) and ethephon (ETH). Virus‐induced gene silencing of CaLRR51 significantly increased the susceptibility of pepper to RSI. By contrast, transient overexpression of CaLRR51 in pepper plants activated hypersensitive response (HR)‐like cell death, and up‐regulated the defence‐related marker genes, including PO2 , HIR1 , PR1 , DEF1 and ACO1 . Moreover, ectopic overexpression of CaLRR51 in transgenic tobacco plants significantly enhanced the resistance to RSI. Transcriptional expression of the corresponding defence‐related marker genes in transgenic tobacco plants was also found to be enhanced by the overexpression of CaLRR51 , which was potentiated by RSI. These loss‐ and gain‐of‐function assays suggest that CaLRR51 acts as a positive regulator in the response of pepper to RSI. In addition, the putative signal peptide and transmembrane region were found to be required for plasma membrane targeting of CaLRR51, which is indispensable for the role of CaLRR51 in plant immunity.