Open AccessHomologues of CsLOB1 in citrus function as disease susceptibility genes in citrus canker
Author(s) -
Zhang Junli,
Huguet Tapia Jose Carlos,
Hu Yang,
Jones Jeffrey,
Wang Nian,
Liu Sanzhen,
White Frank F.
Publication year - 2017
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12441
Subject(s) - biology , citrus canker , gene , canker , genetics , disease , function (biology) , rutaceae , botany , bacteria , medicine , pathology
Summary The lateral organ boundary domain (LBD) genes encode a group of plant‐specific proteins that function as transcription factors in the regulation of plant growth and development. Citrus sinensis lateral organ boundary 1 ( CsLOB1 ) is a member of the LBD family and functions as a disease susceptibility gene in citrus bacterial canker (CBC). Thirty‐four LBD members have been identified from the Citrus sinensis genome. We assessed the potential for additional members of LBD genes in citrus to function as surrogates for CsLOB1 in CBC, and compared host gene expression on induction of different LBD genes. Using custom‐designed transcription activator‐like (TAL) effectors, two members of the same clade as CsLOB1 , named CsLOB2 and CsLOB3 , were found to be capable of functioning similarly to CsLOB1 in CBC. RNA sequencing and quantitative reverse transcription‐polymerase chain reaction analyses revealed a set of cell wall metabolic genes that are associated with CsLOB1 , CsLOB2 and CsLOB3 expression and may represent downstream genes involved in CBC.