Open AccessDeep sequencing leads to the identification of eukaryotic translation initiation factor 5A as a key element in Rsv1 ‐mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean
Author(s) -
Chen Hui,
Adam Arsovski Andrej,
Yu Kangfu,
Wang Aiming
Publication year - 2017
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12407
Subject(s) - biology , gene , genetics , microrna , genome , transcriptome , genotype , translation (biology) , small rna , hypersensitive response , virology , rna seq , computational biology , gene expression , plant disease resistance , messenger rna
Summary Rsv1 , a single dominant resistance locus in soybean, confers extreme resistance to the majority of Soybean mosaic virus (SMV) strains, but is susceptible to the G7 strain. In Rsv1 ‐genotype soybean, G7 infection provokes a lethal systemic hypersensitive response (LSHR), a delayed host defence response. The Rsv1 ‐mediated LSHR signalling pathway remains largely unknown. In this study, we employed a genome‐wide investigation to gain an insight into the molecular interplay between SMV G7 and Rsv1 ‐genotype soybean. Small RNA (sRNA), degradome and transcriptome sequencing analyses were used to identify differentially expressed genes (DEGs) and microRNAs (DEMs) in response to G7 infection. A number of DEGs, DEMs and microRNA targets, and the interaction network of DEMs and their target mRNAs responsive to G7 infection, were identified. Knock‐down of one of the identified DEGs, the eukaryotic translation initiation factor 5A (eIF5A), diminished the LSHR and enhanced viral accumulation, suggesting the essential role of eIF5A in the G7‐induced, Rsv1 ‐mediated LSHR signalling pathway. This work provides an in‐depth genome‐wide analysis of high‐throughput sequencing data, and identifies multiple genes and microRNA signatures that are associated with the Rsv1 ‐mediated LSHR.