Open AccessSuppression of bacterial infection in rice by treatment with a sulfated peptide
Author(s) -
Wei Tong,
Chern Mawsheng,
Liu Furong,
Ronald Pamela C.
Publication year - 2016
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12368
Subject(s) - xanthomonas oryzae , biology , xanthomonas oryzae pv. oryzae , inoculation , microbiology and biotechnology , xanthomonas , plant disease resistance , peptide , gene , pathogen , horticulture , biochemistry
Summary The rice XA21 receptor kinase confers robust resistance to bacterial blight disease caused by Xanthomonas oryzae pv. oryzae ( Xoo ). A tyrosine‐sulfated peptide from Xoo , called RaxX, triggers XA21‐mediated immune responses, including the production of ethylene and reactive oxygen species and the induction of defence gene expression. It has not been tested previously whether these responses confer effective resistance to Xoo . Here, we describe a newly established post‐inoculation treatment assay that facilitates investigations into the effect of the sulfated RaxX peptide in planta . In this assay, rice plants were inoculated with a virulent strain of Xoo and then treated with the RaxX peptide 2 days after inoculation. We found that post‐inoculation treatment of XA21 plants with the sulfated RaxX peptide suppresses the development of Xoo infection in XA21 rice plants. The treated plants display restricted lesion development and reduced bacterial growth. Our findings demonstrate that exogenous application of sulfated RaxX activates XA21‐mediated immunity in planta , and provides a potential strategy for the control of bacterial disease in the field.