Open AccessThiamine‐induced priming against root‐knot nematode infection in rice involves lignification and hydrogen peroxide generation
Author(s) -
Huang WenKun,
Ji HongLi,
Gheysen Godelieve,
Kyndt Tina
Publication year - 2016
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12316
Subject(s) - thiamine , biology , phenylpropanoid , nematode infection , hydrogen peroxide , root knot nematode , microbiology and biotechnology , nematode , phenylalanine ammonia lyase , biochemistry , heterodera schachtii , gene , biosynthesis , phenylalanine , ecology , amino acid
Summary Thiamine (vitamin B 1, VB 1) can act as a plant defence trigger, or priming agent, leading to a rapid counterattack on pathogen invasion. In this study, the priming effect of thiamine on rice ( O ryza sativa cv. N ipponbare) and its activity against root‐knot nematode ( M eloidogyne graminicola ) infection were evaluated. Thiamine treatment and subsequent nematode inoculation activated hydrogen peroxide ( H 2 O 2 ) accumulation and lignin deposition in plant roots, and this correlated with enhanced transcription of OsPAL 1 and OsC4H , two genes involved in the phenylpropanoid pathway. The number of nematodes in rice roots was slightly but significantly reduced, and the development of the nematodes was delayed, whereas no direct toxic effects of VB 1 on nematode viability and infectivity were observed. The combined application of thiamine with l ‐2‐aminooxy‐3‐phenylpropionic acid ( AOPP ), an inhibitor of phenylalanine ammonia‐lyase ( PAL ), significantly hampered the VB 1‐priming capacity. These findings indicate that thiamine‐induced priming in rice involves H 2 O 2 and phenylpropanoid‐mediated lignin production, which hampers nematode infection. Further cellular and molecular studies on the mechanism of thiamine‐induced defence will be useful for the development of novel nematode control strategies.