Open AccessPlant phenolic acids affect the virulence of P ectobacterium aroidearum and P . carotovorum ssp. brasiliense via quorum sensing regulation
Author(s) -
Joshi Janak Raj,
Burdman Saul,
Lipsky Alexander,
Yariv Shaked,
Yedidia Iris
Publication year - 2016
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12295
Subject(s) - virulence , quorum sensing , pectobacterium carotovorum , biology , homoserine , microbiology and biotechnology , gene , bacteria , gene expression , biochemistry , genetics
Summary Several studies have reported effects of the plant phenolic acids cinnamic acid ( CA ) and salicylic acid ( SA ) on the virulence of soft rot enterobacteria. However, the mechanisms involved in these processes are not yet fully understood. Here, we investigated whether CA and SA interfere with the quorum sensing ( QS ) system of two P ectobacterium species, P . aroidearum and P . carotovorum ssp. brasiliense , which are known to produce N ‐acyl‐homoserine lactone ( AHL ) QS signals. Our results clearly indicate that both phenolic compounds affect the QS machinery of the two species, consequently altering the expression of bacterial virulence factors. Although, in control treatments, the expression of QS ‐related genes increased over time, the exposure of bacteria to non‐lethal concentrations of CA or SA inhibited the expression of QS genes, including expI , exp R , PC 1_1442 ( lux R transcriptional regulator) and luxS (a component of the AI ‐2 system). Other virulence genes known to be regulated by the QS system, such as pec S , pel , peh and yhe O , were also down‐regulated relative to the control. In agreement with the low levels of expression of exp I and exp R , CA and SA also reduced the level of the AHL signal. The effects of CA and SA on AHL signalling were confirmed in compensation assays, in which exogenous application of N ‐(β‐ketocaproyl)‐ l ‐homoserine lactone (e AHL ) led to the recovery of the reduction in virulence caused by the two phenolic acids. Collectively, the results of gene expression studies, bioluminescence assays, virulence assays and compensation assays with e AHL clearly support a mechanism by which CA and SA interfere with Pectobacterium virulence via the QS machinery.