Open AccessInvolvement of BcS tr2 in methionine biosynthesis, vegetative differentiation, multiple stress tolerance and virulence in B otrytis cinerea
Author(s) -
Shao Wenyong,
Yang Yalan,
Zhang Yu,
Lv Chiyuan,
Ren Weichao,
Chen Changjun
Publication year - 2016
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12292
Subject(s) - virulence , biology , methionine , biosynthesis , botany , microbiology and biotechnology , genetics , gene , amino acid
Summary The S tr2 gene encodes a cystathionine γ‐synthase that is a key enzyme in methionine ( M et) biosynthesis in S accharomyces cerevisiae . Met plays a critical role in protein synthesis and diverse cellular processes in both eukaryotes and prokaryotes. In this study, we characterized the S tr2 orthologue gene BcS tr2 in B otrytis cinerea . The BcS tr2 mutant was unable to grow on minimal medium ( MM ). In addition, conidia of the mutant were unable to germinate in water–agar medium within 15 h of incubation. Supplementation with 1 m m M et or 0.5 mg/mL homocysteine, but not 1 m m cysteine or 0.5 mg/mL glutathione, rescued the defect in mycelial growth of the B c S tr2 deletion mutant. These results indicate that the enzyme encoded by B c S tr2 is involved in the conversion of cysteine into homocysteine. The mutant exhibited decreased conidiation and impaired sclerotium development. In addition, the BcS tr2 mutant exhibited increased sensitivity to osmotic and oxidative stresses, cell wall‐damaging agents and thermal stress. The mutant demonstrated dramatically decreased virulence on host plant tissues. All of the defects were restored by genetic complementation of the mutant with wild‐type BcS tr2 . Taken together, the results of this study indicate that BcS tr2 plays a critical role in the regulation of various cellular processes in B . cinerea .