Open AccessThe durably resistant rice cultivar D igu activates defence gene expression before the full maturation of M agnaporthe oryzae appressorium
Author(s) -
Li Weitao,
Liu Ya,
Wang Jing,
He Min,
Zhou Xiaogang,
Yang Chao,
Yuan Can,
Wang Jichun,
Chern Mawsheng,
Yin Junjie,
Chen Weilan,
Ma Bingtian,
Wang Yuping,
Qin Peng,
Li Shigui,
Ronald Pamela,
Chen Xuewei
Publication year - 2016
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12286
Subject(s) - biology , appressorium , gene , transcriptome , magnaporthe , pyricularia , oryza sativa , microbiology and biotechnology , gene expression , genetics , botany , magnaporthe grisea
Summary Rice blast caused by the fungal pathogen M agnaporthe oryzae is one of the most destructive diseases worldwide. Although the rice– M . oryzae interaction has been studied extensively, the early molecular events that occur in rice before full maturation of the appressorium during M . oryzae invasion are unknown. Here, we report a comparative transcriptomics analysis of the durably resistant rice variety D igu and the susceptible rice variety Lijiangxintuanheigu ( LTH ) in response to infection by M . oryzae (5, 10 and 20 h post‐inoculation, prior to full development of the appressorium). We found that the transcriptional responses differed significantly between these two rice varieties. Gene ontology and pathway analyses revealed that many biological processes, including extracellular recognition and biosynthesis of antioxidants, terpenes and hormones, were specifically activated in Digu shortly after infection. Forty‐eight genes encoding receptor kinases ( RK s) were significantly differentially regulated by M . oryzae infection in Digu. One of these genes, LOC _Os08g10300 , encoding a leucine‐rich repeat RK from the LRR VIII ‐2 subfamily, conferred enhanced resistance to M . oryzae when overexpressed in rice. Our study reveals that a multitude of molecular events occur in the durably resistant rice Digu before the full maturation of the appressorium after M . oryzae infection and that membrane‐associated RKs play important roles in the early response.