Open AccessMoTup1 is required for growth, conidiogenesis and pathogenicity of M agnaporthe oryzae
Author(s) -
Chen Yue,
Zhai Su,
Sun Yi,
Li Mengying,
Dong Yanhan,
Wang Xiaoli,
Zhang Haifeng,
Zheng Xiaobo,
Wang Ping,
Zhang Zhengguang
Publication year - 2015
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12235
Subject(s) - appressorium , biology , mutant , conidiation , fungal protein , microbiology and biotechnology , gene , repressor , hypha , gene expression , genetics
Summary The general transcriptional repressor Tup1 proteins play important regulatory roles in the growth and development of fungi. In this report, we characterized MoTup1, a protein homologous to Tup1 of S accharomyces cerevisiae , from M . oryzae . Disruption of MoTUP1 resulted in severe mycelial growth reduction and a defect in conidiogenesis. We found that MoTup1 is required for the maintenance of cell wall integrity by regulating the expression of the genes involved in cell wall biosynthesis. Pathogenicity assays indicated that the Δ Motup1 mutants lost the ability to invade both rice and barley hosts. Moreover, observation of rice epidermis penetration showed that the hyphal tips of the mutants could still form appressorium‐like structures, but were unable to invade host cells. Taken together, our results demonstrate that M . oryzae MoTup1 is an important regulatory factor in fungal growth, development and pathogenesis on hosts.