Open AccessRice stripe tenuivirus p2 may recruit or manipulate nucleolar functions through an interaction with fibrillarin to promote virus systemic movement
Author(s) -
Zheng Luping,
Du Zhenguo,
Lin Chen,
Mao Qianzhuo,
Wu Kangcheng,
Wu Jianguo,
Wei Taiyun,
Wu Zujian,
Xie Lianhui
Publication year - 2015
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12220
Subject(s) - fibrillarin , biology , bimolecular fluorescence complementation , movement protein , nicotiana benthamiana , virology , nucleolus , subgenomic mrna , gene silencing , virus , microbiology and biotechnology , cytoplasm , genetics , gene , rna , coat protein
Summary Rice stripe virus ( RSV ) is the type species of the genus T enuivirus and represents a major viral pathogen affecting rice production in E ast A sia. In this study, RSV p2 was fused to yellow fluorescent protein (p2‐ YFP ) and expressed in epidermal cells of N icotiana benthamiana . p2‐ YFP fluorescence was found to move to the nucleolus initially, but to leave the nucleolus for the cytoplasm forming numerous distinct bright spots there at later time points. A bimolecular fluorescence complementation ( BiFC ) assay showed that p2 interacted with fibrillarin and that the interaction occurred in the nucleus. Both the nucleolar localization and cytoplasmic distribution of p2‐ YFP fluorescence were affected in fibrillarin‐silenced N . benthamiana . Fibrillarin depletion abolished the systemic movement of RSV , but not that of T obacco mosaic virus ( TMV ) and Potato virus X ( PVX ). A T obacco rattle virus ( TRV )‐based virus‐induced gene silencing ( VIGS ) method was used to diminish RSV NS2 (encoding p2) or NS3 (encoding p3) during RSV infection. Silencing of NS3 alleviated symptom severity and reduced RSV accumulation, but had no obvious effects on virus movement and the timing of symptom development. However, silencing of NS2 abolished the systemic movement of RSV . The possibility that RSV p2 may recruit or manipulate nucleolar functions to promote virus systemic infection is discussed.