Open AccessA pathogenicity determinant maps to the N ‐terminal coat protein region of the P epino mosaic virus genome
Author(s) -
DuffFarrier Celia R. A.,
Bailey Andy M.,
Boonham Neil,
Foster Gary D.
Publication year - 2015
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12184
Subject(s) - nicotiana benthamiana , biology , solanum , virulence , genome , virology , genotype , genetics , virus , potexvirus , phenotype , gene , coat protein , botany , rna
Summary P epino mosaic virus ( PepMV ) poses a worldwide threat to the tomato industry. Considerable differences at the genetic level allow for the distinction of four main genotypic clusters; however, the basis of the phenotypic outcome is difficult to elucidate. This work reports the generation of wild‐type PepMV infectious clones of both EU (mild) and CH2 (aggressive) genotypes, from which chimeric infectious clones were created. Phenotypic analysis in three solanaceous hosts, N icotiana benthamiana , D atura stramonium and S olanum lycopersicum , indicated that a PepMV pathogenicity determinant mapped to the 3′‐terminal region of the genome. Increased aggression was only observed in N . benthamiana , showing that this factor is host specific. The determinant was localized to amino acids 11–26 of the N ‐terminal coat protein ( CP ) region; this is the first report of this region functioning as a virulence factor in PepMV .