Open AccessNecrotrophic effector‐triggered susceptibility ( NETS ) underlies the barley– P yrenophora teres f. teres interaction specific to chromosome 6H
Author(s) -
Liu Zhaohui,
Holmes Danielle J.,
Faris Justin D.,
Chao Shiaoman,
Brueggeman Robert S.,
Edwards Michael C.,
Friesen Timothy L.
Publication year - 2015
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12172
Subject(s) - pathosystem , biology , quantitative trait locus , population , hordeum vulgare , genetics , locus (genetics) , plant disease resistance , chromosome , inoculation , microbiology and biotechnology , pathogen , botany , horticulture , poaceae , gene , demography , sociology
Summary Barley net form net blotch ( NFNB ), caused by the necrotrophic fungus P yrenophora teres f. teres , is a destructive foliar disease in barley‐growing regions worldwide. Little is known about the genetic and molecular basis of this pathosystem. Here, we identified a small secreted proteinaceous necrotrophic effector ( NE ), designated PttNE1 , from intercellular wash fluids of the susceptible barley line Hector after inoculation with P . teres f. teres isolate 0–1. Using a barley recombinant inbred line (RIL) population developed from a cross between the sensitive/susceptible line Hector and the insensitive/resistant line NDB 112 ( HN population), sensitivity to PttNE1 , which we have named SPN1 , mapped to a common resistance/susceptibility region on barley chromosome 6H . PttNE1 – SPN1 interaction accounted for 31% of the disease variation when the HN population was inoculated with the 0–1 isolate. Strong accumulation of hydrogen peroxide and increased levels of electrolyte leakage were associated with the susceptible reaction, but not the resistant reaction. In addition, the HN RIL population was evaluated for its reactions to 10 geographically diverse P . teres f. teres isolates. Quantitative trait locus ( QTL ) mapping led to the identification of at least 10 genomic regions associated with disease, with chromosomes 3H and 6H harbouring major QTL s for resistance/susceptibility. SPN1 was associated with all the 6H QTL s, except one. Collectively, this information indicates that the barley– P . teres f. teres pathosystem follows, at least partially, an NE‐triggered susceptibility ( NETS ) model that has been described in other necrotrophic fungal disease systems, especially in the D othideomycete class of fungi.