Open AccessThe broad bacterial blight resistance of rice line CBB 23 is triggered by a novel transcription activator‐like ( TAL ) effector of X anthomonas oryzae pv. oryzae
Author(s) -
Wang ChunLian,
Qin TengFei,
Yu HongMan,
Zhang XiaoPing,
Che JinYing,
Gao Ying,
Zheng ChongKe,
Yang Bing,
Zhao KaiJun
Publication year - 2014
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12092
Subject(s) - xanthomonas oryzae , biology , effector , gene , xanthomonas , xanthomonas oryzae pv. oryzae , bacterial blight , activator (genetics) , genetics , oryza sativa , plant disease resistance , cloning (programming) , pathogen , microbiology and biotechnology , computer science , programming language
Summary Bacterial blight ( BB ), caused by X anthomonas oryzae pv. oryzae ( X oo ), is not only a disease devastating rice production worldwide, but also an ideal model system for the study of the interaction between plants and their bacterial pathogens. The rice near‐isogenic line ( NIL ) CBB 23, derived from a cross between a wild rice O ryza rufipogon accession ( RBB 16) and a susceptible indica rice variety ( J ingang 30), is highly resistant to all field X oo strains tested so far. Although the BB resistance of CBB 23 has been widely used in rice breeding programmes, the mechanism of its extremely broad‐spectrum resistance remains unknown. Here, we report the molecular cloning of an avirulence gene, designated as avrXa23 , from X oo strain PXO 99 A . We validate that A vr X a23, a novel transcription activator‐like effector, specifically triggers the broad‐spectrum BB resistance in CBB 23. The prevalence of avrXa23 in all 38 X oo strains surveyed may explain the broad‐spectrum feature of BB resistance in CBB 23. The results will significantly facilitate the molecular cloning of the corresponding resistance ( R ) gene in the host, and provide new insights into our understanding of the molecular mechanism for broad‐spectrum disease resistance in plants.