Open AccessThe target gene of tae‐ miR164 , a novel NAC transcription factor from the NAM subfamily, negatively regulates resistance of wheat to stripe rust
Author(s) -
Feng Hao,
Duan Xiaoyuan,
Zhang Qiong,
Li Xiaorui,
Wang Bing,
Huang Lili,
Wang Xiaojie,
Kang Zhensheng
Publication year - 2014
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12089
Subject(s) - biology , subfamily , transcription factor , gene , stripe rust , genetics , resistance (ecology) , rust (programming language) , plant disease resistance , ecology , computer science , programming language
Summary microRNA ( miRNA ) participates in various physiological and biochemical processes in plants by regulating corresponding target genes. NAC [NAM (no apical meristem), ATAF ( Arabidopsis transcription activation factor) and CUC (cup‐shaped cotyledon)] transcription factors, usually as the targets of miR164 , play important roles in the regulation of plant development and responses to abiotic and biotic stresses. In a previous study, the target gene of tae‐ miR164 in wheat was sequenced through degradome sequencing. In this study, we isolated the full‐length cDNA of the candidate target gene, which is a NAC transcription factor gene in the NAM subfamily, and designated it as TaNAC21/22 after bioinformatics analysis. The interaction between TaNAC21/22 and tae‐ miR164 was confirmed experimentally through co‐transformation of both genes in tobacco leaves. Transcript accumulation of TaNAC21/22 and tae‐miR164 showed contrasting divergent expression patterns in wheat response to P uccinia striiformis f. sp. tritici ( P st ). TaNAC21/22 was confirmed to be located in the nucleus and could function as a transcriptional activator. Silencing of the individual gene showed that TaNAC21/22 negatively regulates resistance to stripe rust. These results indicate that the target of tae‐ miR164 , a novel NAC transcription factor from the NAM subfamily of wheat, plays an important role in regulating the resistance of host plants to stripe rust.