Open AccessTranscriptomic characterization of two major F usarium resistance quantitative trait loci ( QTL s), F hb1 and Q fhs.ifa‐ 5A , identifies novel candidate genes
Author(s) -
Schweiger Wolfgang,
Steiner Barbara,
Ametz Christian,
Siegwart Gerald,
Wiesenberger Gerlinde,
Berthiller Franz,
Lemmens Marc,
Jia Haiyan,
Adam Gerhard,
Muehlbauer Gary J.,
Kreil David P.,
Buerstmayr Hermann
Publication year - 2013
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12048
Subject(s) - quantitative trait locus , transcriptome , biology , genetics , gene , gene expression
Summary Fusarium head blight, caused by F usarium graminearum , is a devastating disease of wheat. We developed near‐isogenic lines ( NILs ) differing in the two strongest known F . graminearum resistance quantitative trait loci ( QTL s), Q fhs.ndsu‐ 3BS (also known as resistance gene Fhb1 ) and Q fhs.ifa‐ 5A , which are located on the short arm of chromosome 3B and on chromosome 5A , respectively. These NILs showing different levels of resistance were used to identify transcripts that are changed significantly in a QTL ‐specific manner in response to the pathogen and between mock‐inoculated samples. After inoculation with F . graminearum spores, 16 transcripts showed a significantly different response for Fhb1 and 352 for Qfhs .ifa‐ 5A . Notably, we identified a lipid transfer protein which is constitutively at least 50‐fold more abundant in plants carrying the resistant allele of Qfhs .ifa‐ 5A . In addition to this candidate gene associated with Qfhs .ifa‐ 5A , we identified a uridine diphosphate ( UDP )‐glycosyltransferase gene, designated TaUGT12887 , exhibiting a positive difference in response to the pathogen in lines harbouring both QTL s relative to lines carrying only the Qfhs .ifa‐ 5A resistance allele, suggesting Fhb1 dependence of this transcript. Yet, this dependence was observed only in the NIL with already higher basal resistance. The complete cDNA of TaUGT12887 was reconstituted from available wheat genomic sequences, and a synthetic recoded gene was expressed in a toxin‐sensitive strain of S accharomyces cerevisiae . This gene conferred deoxynivalenol resistance, albeit much weaker than that observed with the previously characterized barley HvUGT13248 .