Open AccessTa EIL1 , a wheat homologue of At EIN3 , acts as a negative regulator in the wheat–stripe rust fungus interaction
Author(s) -
Duan Xiaoyuan,
Wang Xiaojie,
Fu Yanping,
Tang Chunlei,
Li Xiaorui,
Cheng Yulin,
Feng Hao,
Huang Lili,
Kang Zhensheng
Publication year - 2013
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12044
Subject(s) - biology , haustorium , gene silencing , gene , hypersensitive response , nicotiana benthamiana , transcription factor , complementary dna , plant disease resistance , salicylic acid , microbiology and biotechnology , gene expression , transcription (linguistics) , virulence , genetics , host (biology) , linguistics , philosophy
Summary Transcription factors ( TFs ) play crucial roles in the transcriptional regulation of plant development and defence responses. Increasing evidence has implicated ETHYLENE INSENSITIVE3 ( EIN3 ) in the plant defence response to pathogen infection and environmental stimuli. However, the role of EIN3 in wheat resistance to P uccinia striiformis f. sp. tritici ( Pst ) is not clear. Here, TaEIL1 was isolated by rapid amplification of c DNA ends ( RACE ) based on a sequence fragment from a wheat– Pst interaction c DNA library. The Ta EIL1 protein contains a typical EIN 3‐binding domain, and transient expression analyses indicated that Ta EIL1 is localized in the nucleus. Yeast one‐hybrid assay revealed that Ta EIL1 exhibits transcriptional activity, and its C ‐terminus is necessary for the activation of transcription. TaEIL1 transcripts were regulated by environmental stress stimuli and were decreased under salicylic acid ( SA ) treatment. When wheat leaves were challenged with P st , the transcript level of TaEIL1 in the compatible interaction was approximately three times higher than that in the incompatible interaction. Knocking down TaEIL1 through the Barley stripe mosaic virus ( BSMV ) virus‐induced gene silencing ( VIGS ) system attenuated the growth of P st , with shortened hyphae and reduced hyphal branches, haustorial mother cells and colony size. Moreover, enhanced necrosis was triggered by the P st avirulent race CYR23 , indicating that the hypersensitive response was strengthened in TaEIL1 ‐silenced wheat plants. Thus, the up‐regulation of defence‐related genes and increased sucrose abundance might contribute to the enhanced disease resistance of wheat to the virulent race CYR 31. Taken together, the results suggested that the suppression of TaEIL1 transcripts could enhance the resistance of wheat to stripe rust fungus.