Open AccessQuantitative trait loci for partial resistance to P seudomonas syringae pv. maculicola in A rabidopsis thaliana
Author(s) -
Rant Jenni C.,
Arraiano Lia S.,
Chabannes Matthieu,
Brown James K. M.
Publication year - 2013
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.12043
Subject(s) - pseudomonas syringae , biology , genetics , gene
Summary Segregation of partial resistance to P seudomonas syringae pv. maculicola ( P sm ) ES 4326 was studied in the recombinant inbred population created from accessions (ecotypes) C olumbia ( C ol‐4), the more susceptible parent, and L andsberg ( L er‐0). Plants were spray inoculated with lux‐transformed bacteria in experiments to measure susceptibility. The amount of disease produced on a range of C ol × L er lines by spray inoculation was highly correlated with that produced by pressure infiltration of bacteria into the apoplast. Quantitative trait locus ( QTL ) analysis identified four loci that contributed to partial resistance: QR ps. JIC ‐1.1 , QR ps. JIC ‐2.1 , QR ps. JIC ‐3.1 and QR ps. JIC ‐5.1 on chromosomes 1, 2, 3 and 5, respectively . QR ps. JIC ‐3.1 , located 8.45 cM from the top of the consensus genetic map of chromosome 3, had a large, approximately additive effect on partial resistance, explaining 50% of the genetic variation in this population. Fine mapping narrowed the region within which this QTL was located to 62 genes. A list of candidate genes included several major classes of resistance gene.