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Sirtuin GdSir2.4 participates in the regulation of rRNA transcription in the Giardia duodenalis parasite
Author(s) -
LagunasRangel Francisco Alejandro,
Yee Janet,
BazánTejeda María Luisa,
GarcíaVilla Enrique,
BermúdezCruz Rosa María
Publication year - 2021
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.14710
Subject(s) - biology , giardia lamblia , giardia , nucleolus , epigenetics , chromatin , ribosomal rna , parasite hosting , ribosome biogenesis , genetics , ribosome , gene silencing , gene , rna , world wide web , cytoplasm , computer science
Abstract Giardia duodenalis is a parasite of great medical interest due to the number of infections it causes worldwide each year. Although research on epigenetic mechanisms in this protist has only begun recently, epigenetic regulation has already been shown to have important roles in encystation, antigenic variation, and resistance to antibiotics in Giardia . In this work, we show that a Giardia ortholog of Sir2, GdSir2.4, is involved in the silencing of rRNA expression. Our results demonstrate that GdSir2.4 localizes to the nucleolus, and its binding to the intergenic spacer region of the rDNA is associated with the deacetylation of the chromatin in this region. Given the importance of the regulation of rRNA expression to maintain adequate levels of ribosomes and genomic stability within the cells, GdSir2.4 can be considered a target to create new therapeutic agents against this parasite.