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Gamma carbonic anhydrases are subunits of the mitochondrial complex I of diatoms
Author(s) -
Cainzos Maximiliano,
Marchetti Fernanda,
Popovich Cecilia,
Leonardi Patricia,
Pagnussat Gabriela,
Zabaleta Eduardo
Publication year - 2021
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.14694
Subject(s) - biology , euglena , plastid , chloroplast , diatom , algae , heterotrimeric g protein , protein subunit , red algae , botany , mitochondrion , biochemistry , evolutionary biology , gene , receptor , g protein
Diatoms are unicellular organisms containing red algal‐derived plastids that probably originated as result of serial endosymbioses between an ancestral heterotrophic organism and a red alga or cryptophyta algae from which has only the chloroplast left. Diatom mitochondria are thus believed to derive from the exosymbiont. Unlike animals and fungi, diatoms seem to contain ancestral respiratory chains. In support of this, genes encoding gamma type carbonic anhydrases (CAs) whose products were shown to be intrinsic complex I subunits in plants, Euglena and Acanthamoeba were found in diatoms, a representative of Stramenopiles. In this work, we experimentally show that mitochondrial complex I in diatoms is a large complex containing gamma type CA subunits, supporting an ancestral origin. By using a bioinformatic approach, a complex I integrated CA domain with heterotrimeric subunit composition is proposed.