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Role of mitochondrial complex III/IV in the activation of transcription factor Rst2 in Schizosaccharomyces pombe
Author(s) -
Jiang Guanglie,
Liu Qiannan,
Kato Toshiaki,
Miao Hao,
Gao Xiang,
Liu Kun,
Chen Si,
Sakamoto Norihiro,
Kuno Takayoshi,
Fang Yue
Publication year - 2021
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.14678
Subject(s) - schizosaccharomyces pombe , biology , mitochondrion , transcription factor , microbiology and biotechnology , schizosaccharomyces , reactive oxygen species , biochemistry , transcription (linguistics) , cytoplasm , gene , saccharomyces cerevisiae , linguistics , philosophy
Mitochondria play essential roles in eukaryotic cells for glucose metabolism to produce ATP. In Schizosaccharomyces pombe , transcription factor Rst2 can be activated upon glucose deprivation. However, the link between Rst2 and mitochondrial function remains elusive. Here, we monitored Rst2 transcriptional activity in living cells using a Renilla luciferase reporter system, and found that inhibition of mitochondrial complex III/IV caused cells to produce reactive oxygen species (ROS) and nitric oxide (NO), which in turn activated Rst2. Furthermore, Rst2‐GFP was observed to translocate from cytoplasm to nucleus upon mitochondrial complex III/IV inhibitors treatment, and deletion of genes associated with complex III/IV resulted in delayed process of Rst2‐GFP nuclear exportation under glucose‐rich condition. In particular, we found that Rst2 was phosphorylated following the treatment of complex III/IV inhibitors or SNAP. Altogether, our findings suggest that mitochondrial complex III/IV participates in the activation of Rst2 through ROS and NO generation in Schizosaccharomyces pombe .