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A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage‐derived IncY plasmid
Author(s) -
Kamal Shady Mansour,
CimdinsAhne Annika,
Lee Changhan,
Li Fengyang,
MartínRodríguez Alberto J.,
Seferbekova Zaira,
Afasizhev Robert,
Wami Haleluya Tesfaye,
Katikaridis Panagiotis,
Meins Lena,
Lünsdorf Heinrich,
Dobrindt Ulrich,
Mogk Axel,
Römling Ute
Publication year - 2021
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.14614
Subject(s) - biology , escherichia coli , plasmid , gene , microbiology and biotechnology , genetics , bacteria
The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K‐12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K‐12, the 4.88 Mbp Fec10 genome is characterized by distinct single‐nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two‐component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpG GI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand‐alone disaggregation activity, but observed distinct biochemical characteristics of ClpG GI‐Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpG GI‐SG17M. Furthermore, we noted a unique contribution of ClpG GI‐Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food‐borne heat‐resistant strains in the human gut.