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Calcineurin responsive zinc‐finger‐1 binds to a unique promoter sequence to upregulate neuronal calcium sensor‐1, whose interaction with MID‐1 increases tolerance to calcium stress in Neurospora crassa
Author(s) -
Gohain Dibakar,
Tamuli Ranjan
Publication year - 2019
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.14234
Subject(s) - biology , neurospora crassa , zinc finger , chromatin immunoprecipitation , transcription factor , downregulation and upregulation , calcineurin , microbiology and biotechnology , electrophoretic mobility shift assay , zinc finger transcription factor , promoter , biochemistry , gene expression , gene , medicine , mutant , transplantation , surgery
Summary We studied the molecular mechanism of neuronal calcium sensor‐1 (NCS‐1) signaling pathway for tolerance to Ca 2+ stress in Neurospora crassa . Increasing concentration of Ca 2+ increased the expression of ncs‐1 ; however, the calcineurin inhibitor FK506 severely reduced ncs ‐1 mRNA transcript levels. Chromatin immunoprecipitation (ChIP) studies revealed that the transcription factor calcineurin responsive zinc finger‐1 (CRZ‐1) binds to the ncs‐1 promoter, and CRZ‐1 binding upregulated ncs‐1 expression under high Ca 2+ concentrations. These results suggested the regulation of NCS‐1 function through calcineurin‐ CRZ‐1 signaling pathway. Furthermore, the electrophoretic mobility shift assay (EMSA) revealed that the CRZ‐1 binds specifically to an 8 bp sequence 5ʹ‐CCTTCACA‐3ʹ in the ncs‐1 promoter 216 bp upstream of the ATG start codon. We also showed that NCS‐1 binds to the Ca 2+ permeable channel MID‐1 for tolerance to Ca 2+ stress. Therefore, CRZ‐1 binds to a unique sequence in the ncs‐1 promoter, causing upregulation of NCS‐1 that binds to MID‐1 for tolerance to Ca 2+ stress.