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Complex polar machinery required for proper chromosome segregation in vegetative and sporulating cells of Bacillus subtilis
Author(s) -
Kloosterman Tomas G.,
Lenarcic Rok,
Willis Clare R.,
Roberts David M.,
Hamoen Leendert W.,
Errington Jeff,
Wu Ling J.
Publication year - 2016
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.13393
Subject(s) - biology , bacillus subtilis , cell division , genetics , chromosome segregation , microbiology and biotechnology , mutant , cell cycle , regulator , chromosome , cell cycle protein , cell , gene , bacteria
Summary Chromosome segregation is an essential process of cell multiplication. In prokaryotes, segregation starts with the newly replicated sister origins of replication, oriCs , which move apart to defined positions in the cell. We have developed a genetic screen to identify mutants defective in placement of oriC during spore development in the Gram‐positive bacterium Bacillus subtilis . In addition to the previously identified proteins Soj and DivIVA, our screen identified several new factors involved in polar recruitment of oriC : a reported regulator of competence ComN, and the regulators of division site selection MinD and MinJ. Previous work implicated Soj as an important regulator of oriC positioning in the cell. Our results suggest a model in which the DivIVA‐interacting proteins ComN and MinJ recruit MinD to the cell pole, and that these proteins work upstream of Soj to enable oriC placement. We show that these proteins form a polar complex, which acts in parallel with but distinct from the sporulation‐specific RacA pathway of oriC placement, and also functions during vegetative growth. Our study further shows that MinD has two distinct cell cycle roles, in cell division and chromosome segregation, and highlights that cell probably use multiple parallel mechanisms to ensure accurate chromosome segregation.