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Identification of host genes that affect acquisition of an integrative and conjugative element in B acillus subtilis
Author(s) -
Johnson Christopher M.,
Grossman Alan D.
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.13282
Subject(s) - biology , transposable element , grossman , transformation (genetics) , gene , identification (biology) , genetics , computational biology , botany , genome , keynesian economics , economics
In the article by Johnson and Grossman (2014) there is an error in the description of how competent B. subtilis cells were prepared during the creation of a transposon insertion library. The error is in the left hand column on p. 1296, in the first paragraph of the section titled ‘Competent cell preparation and library transformation’. The recipe for Base medium should read ‘2.25 mM MgSO4’ rather than ‘22.5 mM MgSO4’.