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Premium G‐protein coupled receptor‐mediated nutrient sensing and developmental control in A spergillus nidulans
Author(s)
Brown Neil Andrew,
dos Reis Thaila Fernanda,
Ries Laure Nicolas Annick,
Caldana Camila,
Mah JaeHyung,
Yu JaeHyuk,
Macdonald Jeffrey M.,
Goldman Gustavo Henrique
Publication year2015
Publication title
molecular microbiology
Resource typeJournals
PublisherWiley-Blackwell
Summary Nutrient sensing and utilisation are fundamental for all life forms. As heterotrophs, fungi have evolved a diverse range of mechanisms for sensing and taking up various nutrients. Despite its importance, only a limited number of nutrient receptors and their corresponding ligands have been identified in fungi. G ‐protein coupled receptors ( GPCRs ) are the largest family of transmembrane receptors. The A spergillus nidulans genome encodes 16 putative GPCRs , but only a few have been functionally characterised. Our previous study showed the increased expression of an uncharacterised putative GPCR , gpr H , during carbon starvation. GprH appears conserved throughout numerous filamentous fungi. Here, we reveal that GprH is a putative receptor involved in glucose and tryptophan sensing. The absence of GprH results in a reduction in c AMP levels and PKA activity upon adding glucose or tryptophan to starved cells. GprH is pre‐formed in conidia and is increasingly active during carbon starvation, where it plays a role in glucose uptake and the recovery of hyphal growth. GprH also represses sexual development under conditions favouring sexual fruiting and during carbon starvation in submerged cultures. In summary, the GprH nutrient‐sensing system functions upstream of the c AMP ‐ PKA pathway, influences primary metabolism and hyphal growth, while represses sexual development in A . nidulans .
Subject(s)aspergillus nidulans , biochemistry , biology , ecology , g protein coupled receptor , gene , microbiology and biotechnology , mutant , nutrient , nutrient sensing , receptor , signal transduction , transmembrane protein
Language(s)English
SCImago Journal Rank1.857
H-Index247
eISSN1365-2958
pISSN0950-382X
DOI10.1111/mmi.13135

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