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Molecular biology research to benefit patients with E ntamoeba histolytica infection
Author(s) -
Watanabe Koji,
Petri William A.
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.13131
Subject(s) - biology , axenic , entamoeba histolytica , pathogenesis , colitis , microbiology and biotechnology , immunology , parasite hosting , virology , bacteria , genetics , world wide web , computer science
Summary The development of molecular microbiology has made it possible for us to deepen our understanding of the pathogenesis of amebiasis. Research using the trophozoite form of E ntamoeba histolytica has clearly shown us the importance of the interface between the parasite and host cells in vitro . Immuno‐pathogenesis after excystation was similarly well advanced by the use of a novel murine model of amebic colitis. However, it is still challenging to apply these findings to clinical and epidemiological settings. This is mainly because of the lack of a complete infection animal model of amebiasis by oral–fecal infection. Moreover, in vitro experiments have predominantly been performed using the same axenic cultured strain HM ‐1: IMSS isolated about 50 years ago, whereas highly diverse strains are prevalent all over the world. Translational research informed by clinical observations has the greatest potential for the development of effective interventions. Here, we highlight discoveries of the experiments designed from cohort observation and discuss remaining problems to be solved.