Spatially directed secretion in higher filamentous fungi supports the development of a structure called the Spitzenkörper, through which membrane flows to the hyphal apex. Two flippases, DnfA and DnfB, function in polar growth of hyphae of Aspergillus nidulans, and possibly localize to distinct secretory vesicles, as demonstrated by spatial separation in growing cells. Fully developed A. nidulans conidiophores were here imaged using bright field (top left), with DnfA::GFP (top right), DnfB::mCherry (bottom left panel), and with both channels merged (bottom right). For details, see the article by Schultzhaus et al. on pp. 18–32 and the related MicroCommentary by Penalva on pp. 1–6 of this issue.