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A novel protein, R sf1/ P xd1, is critical for the single‐strand annealing pathway of double‐strand break repair in S chizosaccharomyces pombe
Author(s) -
Wang Hanqian,
Zhang Zhanlu,
Zhang Lan,
Zhang Qiuxue,
Zhang Liang,
Zhao Yangmin,
Wang Weibu,
Fan Yunliu,
Wang Lei
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.13001
Subject(s) - biology , schizosaccharomyces pombe , microbiology and biotechnology , computational biology , genetics , yeast , saccharomyces cerevisiae
Summary The process of single‐strand annealing ( SSA ) repairs DNA double‐strand breaks that are flanked by direct repeat sequences through the coordinated actions of a series of proteins implicated in recombination, mismatch repair and nucleotide excision repair ( NER ). Many of the molecular and mechanistic insights gained in SSA repair have principally come from studies in the budding yeast S accharomyces cerevisiae . However, there is little molecular understanding of the SSA pathway in the fission yeast S chizosaccharomyces pombe . To further our understanding of this important process, we established a new chromosome‐based SSA assay in fission yeast. Our genetic analyses showed that, although many homologous components participate in SSA repair in these species indicating that some evolutionary conservation, S aw1 and S lx4 are not principal agents in the SSA repair pathway in fission yeast. This is in marked contrast to the function of S aw1 and S lx4 in budding yeast. Additionally, a novel genus‐specific protein, R sf1/ P xd1, physically interacts with R ad16, S wi10 and S aw1 in vitro and in vivo . We find that R sf1/ P xd1 is not required for NER and demonstrate that, in fission yeast, R sf1/ P xd1, but not S aw1, plays a critical role in SSA recombination.