Premium
Examination of the S taphylococcus aureus nitric oxide reductase (sa NOR ) reveals its contribution to modulating intracellular NO levels and cellular respiration
Author(s) -
Lewis A. M.,
Matzdorf S. S.,
Endres J. L.,
Windham I. H.,
Bayles K. W.,
Rice K. C.
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12962
Subject(s) - biology , intracellular , nitric oxide , mutant , nitrate reductase , respiration , microbiology and biotechnology , biofilm , biochemistry , bacteria , enzyme , gene , botany , genetics , endocrinology
S taphylococcus aureus nitrosative stress resistance is due in part to flavohemoprotein ( H mp). Although hmp is present in all sequenced S . aureus genomes, 37% of analyzed strains also contain nor , encoding a predicted quinol‐type nitric oxide ( NO ) reductase (sa NOR ). DAF‐FM staining of NO ‐challenged wild‐type, nor , hmp and nor hmp mutant biofilms suggested that H mp may have a greater contribution to intracellular NO detoxification relative to sa NOR . However, sa NOR still had a significant impact on intracellular NO levels and complemented NO detoxification in a nor hmp mutant. When grown as NO ‐challenged static (low‐oxygen) cultures, hmp and nor hmp mutants both experienced a delay in growth initiation, whereas the nor mutant's ability to initiate growth was comparable with the wild‐type strain. However, sa NOR contributed to cell respiration in this assay once growth had resumed, as determined by membrane potential and respiratory activity assays. Expression of nor was upregulated during low‐oxygen growth and dependent on SrrAB , a two‐component system that regulates expression of respiration and nitrosative stress resistance genes. High‐level nor promoter activity was also detectable in a cell subpopulation near the biofilm substratum. These results suggest that sa NOR contributes to NO ‐dependent respiration during nitrosative stress, possibly conferring an advantage to nor + strains in vivo .