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Genome‐scale quantitative characterization of bacterial protein localization dynamics throughout the cell cycle
Author(s) -
Kuwada Nathan J.,
Traxler Beth,
Wiggins Paul A.
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12841
Subject(s) - biology , genome , computational biology , dynamics (music) , scale (ratio) , evolutionary biology , bacterial protein , genetics , bacteria , gene , acoustics , physics , quantum mechanics
Summary Bacterial cells display both spatial and temporal organization, and this complex structure is known to play a central role in cellular function. Although nearly one‐fifth of all proteins in E scherichia coli localize to specific subcellular locations, fundamental questions remain about how cellular‐scale structure is encoded at the level of molecular‐scale interactions. One significant limitation to our understanding is that the localization behavior of only a small subset of proteins has been characterized in detail. As an essential step toward a global model of protein localization in bacteria, we capture and quantitatively analyze spatial and temporal protein localization patterns throughout the cell cycle for nearly every protein in E . coli that exhibits nondiffuse localization. This genome‐scale analysis reveals significant complexity in patterning, notably in the behavior of DNA ‐binding proteins. Complete cell‐cycle imaging also facilitates analysis of protein partitioning to daughter cells at division, revealing a broad and robust assortment of asymmetric partitioning behaviors.

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