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Single‐molecule tracking in live V ibrio cholerae reveals that ToxR recruits the membrane‐bound virulence regulator TcpP to the toxT promoter
Author(s) -
Haas Beth L.,
Matson Jyl S.,
DiRita Victor J.,
Biteen Julie S.
Publication year - 2015
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12834
Subject(s) - biology , vibrio cholerae , virulence , cholera toxin , microbiology and biotechnology , mutant , transcription factor , activator (genetics) , transcription (linguistics) , gene , genetics , bacteria , linguistics , philosophy
Summary V ibrio cholerae causes the human disease cholera by producing a potent toxin. The V . cholerae virulence pathway involves an unusual transcription step: the bitopic inner‐membrane proteins TcpP and ToxR activate toxT transcription. As ToxT is the primary direct transcription activator in V . cholerae pathogenicity, its regulation by membrane‐localized activators is key in the disease process. However, the molecular mechanisms by which membrane‐localized activators engage the transcription process have yet to be uncovered in live cells. Here we report the use of super‐resolution microscopy, single‐molecule tracking, and gene knockouts to examine the dynamics of individual TcpP proteins in live V . cholerae cells with < 40 nm spatial resolution on a 50 ms timescale. Single‐molecule trajectory analysis reveals that TcpP diffusion is heterogeneous and can be described by three populations of TcpP motion: one fast, one slow, and one immobile. By comparing TcpP diffusion in wild‐type V . cholerae to that in mutant strains lacking either toxR or the toxT promoter, we determine that TcpP mobility is greater in the presence of its interaction partners than in their absence. Our findings support a mechanism in which ToxR recruits TcpP to the toxT promoter for transcription activation.