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Morphogenes bolA and mreB mediate the photoregulation of cellular morphology during complementary chromatic acclimation in F remyella diplosiphon
Author(s) -
Singh Shailendra P.,
Montgomery Beronda L.
Publication year - 2014
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12649
Subject(s) - mreb , biology , phycobilisome , gene , gene expression , microbiology and biotechnology , genetics , cyanobacteria , escherichia coli , bacteria
Summary Photoregulation of pigmentation during complementary chromatic acclimation ( CCA ) is well studied in F remyella diplosiphon ; however, mechanistic insights into the CCA ‐associated morphological changes are still emerging. F . diplosiphon cells are rectangular under green light ( GL ), whereas cells are smaller and spherical under red light ( RL ). Here, we investigate the role of morphogenes bolA and mreB during CCA using gene expression and gene function analyses. The F. diplosiphon bolA gene is essential as its complete removal from the genome was unsuccessful. Depletion of bolA resulted in slow growth, morphological defects and the accumulation of high levels of reactive oxygen species in a partially segregated Δ bolA strain. Higher expression of bolA was observed under RL and was correlated with lower expression of mreB and mreC genes in wild type. In a Δ rcaE strain that lacks the red‐/green‐responsive RcaE photoreceptor, the expression of bolA and mre genes was altered under both RL and GL . Observed gene expression relationships suggest that mreB and mreC expression is controlled by RcaE ‐dependent photoregulation of bolA expression. Expression of F . diplosiphon bolA and mreB homologues in E scherichia coli demonstrated functional conservation of the encoded proteins. Together, these studies establish roles for bolA and mreB in RcaE ‐dependent regulation of cellular morphology.