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Mapping of active replication origins in vivo in thaum‐ and euryarchaeal replicons
Author(s) -
Pelve Erik A.,
MartensHabbena Willm,
Stahl David A.,
Bernander Rolf
Publication year - 2013
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12382
Subject(s) - biology , replicon , replication (statistics) , genetics , in vivo , computational biology , evolutionary biology , virology , genome , gene
Summary We report mapping of active replication origins in thaum‐ and euryarchaeal replicons using high‐throughput sequencing‐based marker frequency analysis. The chromosome of the thaumarchaeon N itrosopumilus maritimus is shown to contain a single origin of replication, whereas the main chromosome in the halophilic euryarchaea H aloferax mediterranei and H aloferax volcanii each contains two origins. All replication origins specified bidirectional replication, and the two origins in the halophiles were initiated in synchrony. The p HM 500 plasmid of H . mediterranei is shown to contain a single origin, and the copy numbers of five plasmid replicons in the two halophiles were inferred to be close to that of the main chromosome. Origin recognition boxes ( ORB s) that provide binding sites for O rc1/ C dc6 replication initiator proteins are identified at all chromosomal origins, as well as in a range of additional thaumarchaeal species. An annotation update is provided for all three species.