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IS Dra 2 transposition in D einococcus radiodurans is downregulated by TnpB
Author(s) -
Pasternak Cécile,
Dulermo Rémi,
TonHoang Bao,
Debuchy Robert,
Siguier Patricia,
Coste Geneviève,
Chandler Michael,
Sommer Suzanne
Publication year - 2013
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12194
Subject(s) - transposase , transposition (logic) , biology , transposable element , insertion sequence , genetics , heterologous , gene , microbiology and biotechnology , mutant , linguistics , philosophy
Summary Transposable elements belonging to the recently identified IS 200 / IS 605 family radically differ from classical insertion sequences in their transposition mechanism by strictly requiring single‐stranded DNA substrates. This IS family includes elements encoding only the transposase ( TnpA ), and others, like IS Dra 2 from D einococcus radiodurans , which contain a second gene, tnpB , dispensable for transposition and of unknown function to date. Here, we show that TnpB has an inhibitory effect on the excision and insertion steps of IS Dra 2 transposition. This inhibitory action of TnpB was maintained when IS Dra 2 transposition was induced by γ‐irradiation of the host cells and required the integrity of its putative zinc finger motif. We also demonstrate the negative role of TnpB when IS Dra 2 transposition was monitored in a heterologous E scherichia coli host, indicating that TnpB ‐mediated inhibition does not involve D einococcus ‐specific factors. TnpB therefore appears to play a regulatory role in IS Dra 2 transposition.